The predominate method used nowadays for DNA sequencing is an enzymatic technique, known as dideoxy sequencing or the Sanger method (to distinguish it from the chemical Maxam/Gilbert sequencing method
The PCR is an in vitro technique that allows one to clone a stretch of DNA in the test tube, without the necessity of cloning and subcloning in bacteria. In doing so, it has also facilitated a host of
One application of PCR, among many, pertains to its use in the determination of pedigree and uses a form of polymorphic DNA found in so-called minisatellite regions which contain variable numbers of t
Another technique used in pedigree determination is known as RAPD. This is really a stripped-down version of PCR but uses a single sequence in the design of the primer (ie. two primers are still neede
