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+ Generation of DNA Circles in Yeast by Inducible Site-Specific Recombination(generation,dna,circles)
[Generation of DNA Circles in Yeast by Inducible Site-Specific Recombination(generation,dna,circles)] Site-specific recombinases have been harnessed for a variety of genetic manipulations involving the gain, loss, or rearrangement of genomic DNA i [关键词:generation dna circles ]……
标签:generation dna circles


+ Assaying Chromatin Structure and Remodeling by Restriction Enzyme Accessibility(assaying,chromatin,structure)
[Assaying Chromatin Structure and Remodeling by Restriction Enzyme Accessibility(assaying,chromatin,structure)] The packaging of eukaryotic DNA into nucleosomes, the fundamental unit of chromatin, creates a barrier to nuclear processes, such as transcriptio [关键词:assaying chromatin structure ]……
标签:assaying chromatin structure


+ Quantitative Trait Locus Analysis in Haplodiploid Hymenoptera(quantitative,trait,locus)
[Quantitative Trait Locus Analysis in Haplodiploid Hymenoptera(quantitative,trait,locus)] This article describes QTL analyses for solitary (Nasonia , a parasitoid wasp) and social hymenopteran species (honeybee and bumblebee) These ex [关键词:quantitative trait locus ]……
标签:quantitative trait locus


+ Optimizing Saccharomyces cerevisiae Induction Regimes(optimizing,saccharomyces)
[Optimizing Saccharomyces cerevisiae Induction Regimes(optimizing,saccharomyces)] Recombinant membrane protein yields can be optimized in Saccharomyces cerevisiae by adjusting the induction time and temperature and or by the ad [关键词:optimizing saccharomyces ]……
标签:optimizing saccharomyces


+ The Effect of Antifoam Addition on Protein Production Yields(effect,antifoam,addition)
[The Effect of Antifoam Addition on Protein Production Yields(effect,antifoam,addition)] Pichia pastoris is a widely used host for recombinant protein production The foaming associated with culturing it on a large scale is commonly p [关键词:effect antifoam addition ]……
标签:effect antifoam addition


+ Screening for High-Yielding Saccharomyces cerevisiae Clones: Using a Green Fluorescent Protein Fusion Strategy in the Production of Membrane Proteins(screening,high-yielding)
[Screening for High-Yielding Saccharomyces cerevisiae Clones: Using a Green Fluorescent Protein Fusion Strategy in the Production of Membrane Proteins(screening,high-yielding)] The overproduction of eukaryotic membrane proteins in milligram quantities is a major bottleneck for their further biochemical and structural inv [关键词:screening high-yielding ]……
标签:screening high-yielding


+ Measurement of Precursor miRNA in Exosomes from Human ESC-Derived Mesenchymal Stem Cells(measurement,precursor,mirna)
[Measurement of Precursor miRNA in Exosomes from Human ESC-Derived Mesenchymal Stem Cells(measurement,precursor,mirna)] Mesenchymal stem cells (MSCs) derived from human embryonic stem cells (ESCs) have been shown to secrete exosomes that are cardioprotective agains [关键词:measurement precursor mirna ]……
标签:measurement precursor mirna


+ Analysis of MicroRNA and Protein Transfer by Exosomes During an Immune Synapse(analysis,microrna,protein)
[Analysis of MicroRNA and Protein Transfer by Exosomes During an Immune Synapse(analysis,microrna,protein)] Immune cells release microRNA-containing exosomes that can be taken up by recipient cells Exosomes can thus act as mediators of cell–cell commun [关键词:analysis microrna protein ]……
标签:analysis microrna protein


+ Functional Analysis of Exosomal MicroRNA in CellCell Communication Research(functional,analysis,exosomal)
[Functional Analysis of Exosomal MicroRNA in CellCell Communication Research(functional,analysis,exosomal)] Circulating microRNAs (miRNAs), also known as secretory miRNAs, are packaged in small membrane vesicles called exosomes These exosomal miRNAs ar [关键词:functional analysis exosomal ]……
标签:functional analysis exosomal


+ Identification of Prostate Cancer-Associated MicroRNAs in Circulation Using a Mouse Model of Disease(identification,prostate)
[Identification of Prostate Cancer-Associated MicroRNAs in Circulation Using a Mouse Model of Disease(identification,prostate)] MicroRNAs (miRNAs) derived from the cell-free fractions of blood are emerging as useful noninvasive markers of cancer However, many tumors displ [关键词:identification prostate ]……
标签:identification prostate


+ Engineering B Cells with mRNA(cells,mrna)
[Engineering B Cells with mRNA(cells,mrna)] Ex vivo activated B cells are an alternative source of antigen presenting cells (APC) However, the ability of ex vivo activated B cells to funct [关键词:cells mrna ]……
标签:cells mrna


+ Measuring Nucleosome Occupancy In Vivo by Micrococcal Nuclease(measuring,nucleosome,occupancy)
[Measuring Nucleosome Occupancy In Vivo by Micrococcal Nuclease(measuring,nucleosome,occupancy)] Eukaryotic genomes are wrapped in nucleosomes These nucleosomes could be a barrier or could help facilitate the binding of transcription or repl [关键词:measuring nucleosome occupancy ]……
标签:measuring nucleosome occupancy


+ eQTL(eqtl)
[eQTL(eqtl)] The goal of mapping expression quantitative trait loci (eQTLs) is to identify genomic regions regulating gene expression traits, which can be gat [关键词:eqtl ]……
标签:eqtl


+ Analysis of the Transfer of Circulating microRNA Between Cells Mediated by Gap Junction(analysis,transfer,circulating)
[Analysis of the Transfer of Circulating microRNA Between Cells Mediated by Gap Junction(analysis,transfer,circulating)] A significant breakthrough in the field of research was the identification of microRNAs (miRNAs), which are small molecule, single-stranded nucle [关键词:analysis transfer circulating ]……
标签:analysis transfer circulating


+ Analysis of Viral MicroRNA Exchange via Exosomes In Vitro and In Vivo(analysis,viral,microrna)
[Analysis of Viral MicroRNA Exchange via Exosomes In Vitro and In Vivo(analysis,viral,microrna)] The isolation and analysis of microRNAs (miRNAs) contained in microvesicles and in particular nano-sized exosomes has become an increasingly impo [关键词:analysis viral microrna ]……
标签:analysis viral microrna


+ Methods of Analysis of Dendritic Cell-Derived Exosome-Shuttle MicroRNA and Its Horizontal Propagation Between Dendritic Cells(methods,analysis,dendritic)
[Methods of Analysis of Dendritic Cell-Derived Exosome-Shuttle MicroRNA and Its Horizontal Propagation Between Dendritic Cells(methods,analysis,dendritic)] Exosomes are extremely small (<100 nm) membrane vesicles, generated in the endocytic compartment that are released to the extracellular milieu by [关键词:methods analysis dendritic ]……
标签:methods analysis dendritic


+ Isolation of Extracellular Nanovesicle MicroRNA from Liver Cancer Cells in Culture(isolation,extracellular,nanovesicle)
[Isolation of Extracellular Nanovesicle MicroRNA from Liver Cancer Cells in Culture(isolation,extracellular,nanovesicle)] MicroRNA can be transferred across cells within extracellular vesicles such as exosomes In order to analyze the biological effects of extracellu [关键词:isolation extracellular nanovesicle ]……
标签:isolation extracellular nanovesicle


+ A Combination of Extraction Reagent and DNA Microarray That Allows for the Detection of Global MiRNA Profiles from Serum/Plasma(combination,extraction,reagent)
[A Combination of Extraction Reagent and DNA Microarray That Allows for the Detection of Global MiRNA Profiles from Serum Plasma(combination,extraction,reagent)] In recent years, miRNAs have been shown to exist stably in serum (plasma) and have drawn attention particularly as markers for diagnosis of disea [关键词:combination extraction reagent ]……
标签:combination extraction reagent


+ Large Volume Flow Electroporation of mRNA: Clinical Scale Process(large,volume,flow)
[Large Volume Flow Electroporation of mRNA: Clinical Scale Process(large,volume,flow)] Genetic modification for enhancing cellular function has been continuously pursued for fighting diseases Messenger RNA (mRNA) transfection is fo [关键词:large volume flow ]……
标签:large volume flow


+ Programming Human Dendritic Cells with mRNA(programming,human)
[Programming Human Dendritic Cells with mRNA(programming,human)] Transfecting with in vitro transcribed, protein-encoding mRNA is a simple yet effective method to express high levels of the desired RNA-encoded [关键词:programming human ]……
标签:programming human


+ Light-Induced mRNA Transfection(light-induced,light,induced)
[Light-Induced mRNA Transfection(light-induced,light,induced)] mRNA-based transfection is an attractive strategy for manipulation of gene expression for gain-of-function studies and therapeutic applications [关键词:light-induced light induced ]……
标签:light-induced light induced


+ Nonviral, Cationic Lipid-Mediated Delivery of mRNA(nonviral,cationic,lipid-mediated)
[Nonviral, Cationic Lipid-Mediated Delivery of mRNA(nonviral,cationic,lipid-mediated)] Appropriate gene delivery systems are essential for successful gene therapy in clinical medicine Cationic lipid-mediated delivery is an alternat [关键词:nonviral cationic lipid-mediated ]……
标签:nonviral cationic lipid-mediated


+ HPLC Purification of In Vitro Transcribed Long RNA(hplc,purification,vitro)
[HPLC Purification of In Vitro Transcribed Long RNA(hplc,purification,vitro)] In vitro transcription of DNA with phage RNA polymerases is currently the most efficient method to produce long sequence-specific RNA While the [关键词:hplc purification vitro ]……
标签:hplc purification vitro


+ Generation of cDNA Libraries from RNP-Derived Regulatory Noncoding RNAs(generation,cdna,libraries)
[Generation of cDNA Libraries from RNP-Derived Regulatory Noncoding RNAs(generation,cdna,libraries)] Next-generation sequencing of noncoding RNA (ncRNA) libraries has become an essential tool for the profiling of ncRNAs and the identification of [关键词:generation cdna libraries ]……
标签:generation cdna libraries


+ Using Mini-genes to Identify Factors That Modulate Alternative Splicing(using,mini-genes,mini)
[Using Mini-genes to Identify Factors That Modulate Alternative Splicing(using,mini-genes,mini)] Many genetic mutations result in the disruption of (alternative) splicing Prime examples are the SMN1 and SMN2 genes: a silent mutation in SMN2 [关键词:using mini-genes mini ]……
标签:using mini-genes mini


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