自Nat Biotechnol 2001 Oct;19(10):958-61】
4. 病原体的临床病理学角度
Bacteria were the first organisms recognized for their potential as agents of bioaggression and the possibility of their use by a terrorist or rogue nation is considered a significant threat. Five of the more likely agents (anthrax, plague, tularemia, Q fever, and brucellosis) are reviewed with emphasis on their epidemiology, clinical presentation, diagnosis, and pathology. Particular emphasis is given to the presentation of the diseases as they may appear after use in a biowarfare scenario.
【引自Clin Lab Med 2001 Sep;21(3):513-48】
5. 在加拿大北部流行地区的炭疽热孢子体检测
AIMS: To determine the level of anthrax spore contamination in endemic regions of northern Canada between outbreaks. METHODS AND RESULTS: Bacterial endospores were extracted from specimens via flotation and cultured on selective PLET medium. Of 588 environmental specimens collected, 11 (1.9%) contained viable anthrax spores. CONCLUSION: High environmental concentrations of anthrax spores in northern Canada appear limited to scavenger faeces and anthrax carcass sites. Burial and cremation appear equally effective at removing anthrax spores from the immediate environment, though cremation may be improved by re-burning cremation sites containing unburned animal hair. SIGNIFICANCE AND IMPACT OF THE STUDY: This study describes an effective anthrax spore detection system. It provides the first bacteriological evidence that mammalian scavengers can disseminate anthrax spores in northern Canada, and its results may be compared with future environmental studies of untreated anthrax carcass sites to help improve government response plans.
【引自J Appl Microbiol 2001 Sep;91(3):435-41】
6. 肉和组织中炭疽杆菌的简便、敏感的检测系统
AIMS: To detect and isolate Bacillus anthracis from meat and tissue by rapid and simple procedures. METHODS AND RESULTS: Bacillus anthracis Pasteur II cells were added to 1 g lymph node and pig meat, which were then cut into small pieces and suspended in PBS. Aliquots were spread on Bacillus cereus selective agar (BCA) plates to isolate B. anthracis cells, and incubated in trypticase soy broth. The enrichment culture was used for nested PCR with B. anthracis specific primers, which were to confirm the presence of B. anthracis chromosomal DNA and the pXO1/pXO2 plasmids. CONCLUSION: One cell of B. anthracis was detected by nested PCR from 1 g of the samples, and was also isolated on BCA plates according to colony morphology within two days. SIGNIFICANCE AND IMPACT OF THE STUDY: These results could be useful for detecting animals with latent anthrax, and meat contaminated with B. anthracis, rapidly and simply.
【引自J Appl Microbiol 2001 Sep;91(3):421-6】
7. 通过实时PCR检测空气中的炭疽热孢子体
AIMS: To detect and isolate Bacillus anthracis from the air by a simple and rapid procedure. METHODS AND RESULTS: One hundred litres of air were filtered through an air monitor device. A

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