FITC和PI
PI和EB 都具有嵌入到双链DNA和RNA的碱基对中并有与碱基对结合的特异性。为了获得特异的DNA分布，染色前必须用RNA酶处理细胞，排除双链RNA的干扰。PI和EB不能进入完整的细胞膜，因此又可以用于检测死细肥。PI和EB各种理化性质相似，但PI比EB的发射光光谱峰向长波方向移动，因而在作DNA和蛋白质双参数测量时，PI的红色荧光和FITC的绿色荧光更易于区分和测量。另外，Pl比EB测得的DNA分布的变异系数(CV值)低，所以PI得到更广泛的应用。
FITC 为一种小分子荧光素，其效率(即荧光强度)取决于溶液的pH值，因此在使用FITC时应注意溶液的酸碱度。
一些流式常用名字的概念和意义：
Amplifier 放大器 Electronic component of a flow cytometer that increases the signal by an adjustable factor.
Aneuploid 异倍体 Having an abnormal number of chromosomes. Aneuploid cells may also have an abnormal DNA content.
Average The mean value, which is the total amount divided by the number of contributors.
Channel 常说的荧光道数 The measured value of a parameter, representing the signal intensity of an event after amplification. To appear on a plot, data for an event must fall into one of either 256 channels (0-255) or one of 1024 channels (0-1023) depending on the resolution of the plot.
Compensation （荧光）补偿 An electronic calculation that removes signal overlap which the optical system cannot remove. Fluorescence compensation works for specific pairs of fluorescent parameters; for example, FL1 and FL2.
Cursor For FACScans: a highlight appearing in a data field that indicates you can modify this field. On a dot plot, a crosshair for drawing a polygon gate. On other flow cytometers: a line separating regions on single parameter histograms that are treated statistically separate. See marker.
%CV CV值！表示其集中分散趋势。Percent coefficient of variation is a measure of peak distribution. The percent coefficient of variation is the standard deviation of the peak divided by the mean channel number of the peak, times 100.
Diploid 二倍体Having the normal number of chromosome pairs for non-reproductive, mammalian cells. The amount of DNA in diploid cells defines the normal DNA content for the species.
DNA Index The ratio of GO/G1 peak channel in a DNA histogram of the experimental sample to the GO/G1 peak channel of the reference sample, when normal human diploid cells or nuclei are the reference. This is a measure of DNA aneuploidy, or abnormal DNA content.
Dot Plot A two parameter data graph used for acquisition and analysis. Each dot on the display represents one event that the flow cytometer analyzed.
Doublet 分选中常用的概念。 Two particles stuck together, which a flow cytometer records as one larger event. Particles may also occur in triplets and higher associations.
Event A unit of data representing one particle or cell. An event has a relative intensity value and channel number for each parameter.
Filter An optical device used to attenuate particular wavelengths or frequencies while passing others.
FL1, Green Fluorescence signal received by the photomultiplier tube (PMT), FL1. The range of the signal detected is dependent on the filters associated with the PMT. For FACScans and FACSCaliburs, FL1 measures light in the green range of the spectrum (515 to 545 nm).
FL2, Orange-Red Fluorescence signal received by the photomultiplier tube, FL2. For FACScans and FACSCaliburs, FL2 measures orange-red light (564-606 nm).
FL3, Red Fluorescence signal received by the photomultiplier tube, FL3. For FACScans, FL3 measures red light (above 650 nm). For FACSCaliburs, FL3 measures red light (above 670 nm).
FL4, Red-Orange Fluorescence signal received by the photomultiplier tube, FL4. For FACSCaliburs, FL4 measures red-orange light (653-669 nm).
