此外，全血法流式细胞术还可用于检测血小板聚集，研究其免疫表型HPA-Ⅰa，测定严重血小板减少患者的血小板数目。在尿毒症，心肺分流术（体外循环）中，本法也有临床诊断价值。
作者单位：510630 广州，暨南大学华侨医院检验科
参考文献
1 George JN, Shattil SJ. The clinical important of acquired abnormalities of platelet function. N Engl J Med,1991，324:27-39.
2 Shattil SJ, Cunningham M，Hoxie JA，et al. Detection of activated platelets in whole blood using activation-dependent monoclonal antibodies and flow cytometry. Blood，1987,70:307-315.
3 Michelson AD, Ellis PA, Barnand MR, et al. Downregulation of the platelet surface glycoprotein Ib-IX complex in whole blood stimulated by thrombin, ADP or an in vivo wound. Blood, 1991,77:770-779.
4 Michelson AD. Thrombin-induced down-regulation of the platelet membrane glycoprotein Ib-IX complex. Semin Thromb Hemost, 1992,18:18-25.
5 Johnston GL, Pickeet EB, McEver RP, et al. Heterogeneity of platelet secretion in response thrombin demonstrated by fluorescence flow cytometry. Blood, 1987,69:1401-1413.
6 Kestin AS, Ellis PA, Barnard MR, et al. The effect of strenuous exercise on platelet activation state and reactivity. Circulation, 1993,88:1502-1511.
7 Peerschke EIB. Platelet membrane glycoproteins: Functional characterization and clinical applications. Am J Clini Pathol, 1992,98:455-463.
8 Michelson AD. Flow cytometry: A clinical test of platelet function. Blood, 1996,87:4925-4936.
9 Ault KA. Flow cytometric measurement of platelet function and reticulated platelets. Ann NY Acad Sci, 1993,677:293-308.
10 Davies TA, Drotts DJ, Weil GJ, et al. Flow cytometric measurements of cytoplasmic calcium changes in human platelet. Cytometry, 1988,9:138-142.
11 Gordon N, Thom J, Cole C, et al. Rapid detection of hereditary and acquired platelet storage pool deficiency by flow cytometry. Br J Haematol, 1995,89:117-123.
12 Inoun T, Hoshi K, Fujito T, et al. Early detection of platelet activation after coronary angioplastry. Coron Artery Dis, 1996,7:529-534.
13 Pongratz G, Brandt PM, Henneke KH, et al. Platelet activation in embolic and preembolic states of patients with nonrheumatic atrial fibirilation. Chest, 1997,111:929-933.
14 Bunescu A, Lindabl T, Solum NO, et al. Partial expression of GPIb measured by flow cytometry in two patients with Bernard-Soulier syndrome. Thromb Res, 1994,76:441-450.
15 Jennings LK, Ashmun RA, Wang WC, et al. Analysis of human platelet glycoproteins Ⅱb-Ⅲa and Glanzamann′s thrombasthenin in whole blood by flow cytometry. Blood, 1996,68:173-179.
16 Rinder HW, Murphy M, Mitchell JG, et al. Progressive platelet activation with storage: Evidence for shortened survival of activated platelets after transfusion. Transfusion, 1991,31:409-414.
17 Coller BS, Scudder LE, Beer J, et al. Monoclonal antibodies to platelet GPⅡb-Ⅲa as antithrombotic agents. Ann N Y Acad Sci, 1991,614:193-213.
18 Dennis MS, Henzel WJ, Pitti RM, et al. Platelet glycoprotein IIb-IIIa protein antagonists from snake venoms: Evidence of a family of platelet aggregation inhibitors. Proc Natl Acad Sci. USA, 1989.87:2471-2475.
