Introduction
“Flow” + “Cell” + “Measurement” = FCM (FACS®)
What it does:
Measures cell functionalities and quantities (Fluorescence)
Measures cell sizes (scattering)
Sort different cells (very selective)
Applications
Study cellular mechanisms
Viral binding
Gene expression
Immune recognition
Clinical studies
Cancer cell determination
WBC ID (eg CD-4)
Procedure
Procedure
Disperse cells
Tag cells w/ fluorochrome
Antibody
Glycoprotein receptor
Intracellular receptor
Add sheath fluid
Laminar flow
Form “thread” of cells
Up to 1000’s cells/sec
Excite with laser
Detection:
Fluorescence (photodiode array
Light scattering
