The seminar of “Gene engineering”in April 2008

Paper source: JOURNAL OF VIROLOGY, June 2003, Vol. 77, No. 11, p. 6450–6465
Paper tiltle: Development and Application of a Reverse Genetics System for Japanese Encephalitis Virus
Authors: Sang-Im Yun, Seok-Yong Kim, Charles M. Rice, and Young-Min Lee
Mailing address: Department of Internal Medicine, Microbiology, College of Medicine and Medical Research Institute.

Chungbuk National University, Cheongju, Korea, and Center for the Study of Hepatitis C, Laboratory for Virology and Infectious Disease, The Rockefeller University.

Discussion topics:

1. The background of JEV;
2. The background of reverse genetics;
3. The application value of full-length infectious cDNA of virus;
4. Complete nucleotide sequence analysis of JEV genomic RNA;
5. Construction of full-length infectious cDNAs for JEV;
6. Construction of authentic 3 terminus for JEV;
7. Construction of authentic 5 terminus for JEV;
8. Mutagenesis of full-length infectious JEV cDNA;
9. Insertion analysis of foreign genes;
10. Transcriptions analysis of full-length infectious cDNAs for JEV;
11. Transfections analysis of full-length infectious cDNAs for JEV;
12. Stability analysis of Cloned full-length cDNA
13. Conclusion.