RT inhibition of real-time PCR
Take home message –
• 1 tube RT-PCR must use carrier protein(RNase inhibitor, aCry)
• 2 tube RT-PCR can either heat kill or use carrier protein
cDNA synthesis: yield vs. time 42℃
1 ug RNA, 200 units MMLV, random primed β Actin TaqMan assay
cDNA synthesis: yield vs. time 25℃
25℃ Time course, MMLV, β Actin TaqMan assay
cDNA synthesis: 25℃ vs. 42℃
