Step 1 in RNA Isolation: sample handling
Obtain tissue sample
Fresh tissue: can not pause until lysate obtained
or …
Snap Freeze with Liquid N2:
Not possible in many settings
Cumbersome; must carry Dewar
Grinding tissue is laborious and potentially hazardous
Handling and transfer difficult (cross-contamination?)
Processing large fragments is especially problematic
Obtain tissue sample
Fresh tissue: can not pause until lysate obtained(well, not exactly…..)
Obtain tissue sample
Fresh tissue: can not pause until lysate obtained
or …
Snap Freeze with Liquid N2:
Not possible in many settings
Cumbersome; must carry Dewar
Grinding tissue is laborious and potentially hazardous
Handling and transfer difficult (cross-contamination?)
Processing large fragments is especially problematic
Effects of tissue freeze/thaw on RNA quality
