Carrier materials
•Poly sugars
–Sepharose (Agarose), Sephadex (Dextran), Sephacel (Cellulose)
–Rare sugars can hardly be utilised by bacteria.
–Low flow rates
–Material changes it’s Volume depending on the ionic strangth
–Material settles over time
•Beads
–Polystyrol/Divinylbenzen beads with charge carrier added
–Relative high flow rates–Good pressure stability, no compression
–Hard charges could stress the protein
•Beads and tentacle
–Charges are linked with flexible spacers to polymeric beads. This leads to a soft binding of proteins despite of hard charges on the matrix
–Relative high flow rates
–Good pressure stability
–High capaticity
•Perfusion beads
–Porous material, beads with chanels
–Very big surface
–Highly pressure stable
–Very high flow rates
Quality of the separation
