Binding behaviour of proteins
•pI= isoelectricpoint of the protein the pH value at which the posses no net charge
•The pI determines the charge of the protein at a given pH
pH > pI negative net charge
pH < pI positive net charge
pIand protein separation on ion exchange columns
But...
•pIis not alone responsible for the binding behaviour of proteins
Binding is sometimes influenced by local charges and not by thenet charge of the protein
Example for a separation by ion exchange chromatography
Materials
