Size-Exclusion Chromatography
Also called gel filtration: The column matrix is a cross-linked polymer with pores of selected size.
Larger protein migrate faster than smaller ones because they are too large to enter the pores
Affinity Chromatography
Separate protein by their binding specificities. The proteins retained on the column are those that bind specifically to a ligand cross-linked to the beads. Proteins that do not binds to ligands are washed through to column
Electrophoresis
