Buffers and solutions:
Transblot* buffer for wet transfer:
12.1 g Tris
57.7 g Glycine
800 ml MeOH
QS to 4 liters with DI water.
Transblot** buffer for continuous semidry transfer:
39 mM Glycine 2.93g
48 mM Tris 5.81g
0.03% w/v SDS 0.375 g
20% MeOH 200 ml
QS to 1 liter with DI water.
10 X TBS
1.5 M NaCl 87.6 g
100 mM Tris pH 7.5 12.1 g
QS to 1000 mL with water.
Laemmli Lower Tris Buffer (4X)
1.5 M tris 90.83 g tris base
0.4 % SDS 20.00 ml of 10 % SDS stock
QS to 500 ml Water
PH with HCl to 8.8
Laemmli Upper Tris Buffer (4X)
0.5 M tris 30.28 g tris base
0.4 % SDS 20.00 ml of 10% SDS stock
QS to 500 ml
PH with HCl to 6.8
Laemmli Reservoir Buffer (4X) 10X
Tris base 12 g 30 g
Glycine 57.6 g 144 g
10 % SDS 40 ml 100 ml
QS to 1000 ml
PH with HCl to 8.3
Laemmli Sample bufffer (4X)
Glycerol 1.0ml
10 % SDS 3.0 ml
4X upper tris 1.25 ml
bromophenol blue 0.210 ml of 1%
ADD BME to 20 % just prior to use!!.
Lower SDS PAGE GEL for Western Blot
Lower SDS PAGE GEL for Western Blot
