Western Blotting (WB)
WB is a protein detection technique that combines the separation power of SDS PAGE together with high recognition specificity of antibodies
An antibody against the target protein could be purified from serum of animals (mice, rabbits, goats) immunized with this protein
Alternatively, if protein contains a commonly used tag or epitope, an antibody against the tag/epitope could be purchase from a commercial source (e.g. anti-6 His antibody)
WB: 4 steps
1.Separation of proteins using SDS PAGE
2. Transfer of the proteins onto e.g. a nitrocellulose membrane (blotting)
3. Immune reactions
4. Visualization
WB, Step 2: Blotting
WB, Steps 3-4: Detection
