Mixtures of ampholytes, small amphoteric molecules with high buffering capacity near their pI, are used to generate the pH gradient.
Positively and negatively charged proteins move to – and +, respectively, until they reach pI.
PI of proteins can be theoretically predicted. Therefore, IEF can also be used for protein identification.
IEF example
2D PAGE
2D PAGE
Lung V79 cells from chinese hamster
Guillermo Senisterra Dept. of Physics-University of Waterloo-Waterloo-Ontario N2L 3G1-Canada
