In the absence of phospholipids, both twinfilins run as a single sharp band on this gel. PI(4,5)P2 causes twinfilin-1 and twinfilin-2 to move more rapidly toward the anode, indicating a net increase in the negative charge and thus a binding interaction. Vartiainen et al. JBC 2003
Dimerization of KIR2DL1 in the presence of Co2 . Qing R. Fan et al. JBC 2000
Native gradient PAGE
Separate native proteins by size – proteins stop moving when they reach a sertain gel density (but this may take a very long time ...)
A great technique to study protien oligomerization!
Native gradient PAGE example
Urea PAGE
