Proteomics Tools
Molecular Biology Tools
Separation & Display Tools
Protein Identification Tools
Protein Structure Tools
Separation & Display Tools
1D Slab Gel Electrophoresis
2D Gel Electrophoresis
Capillary Electrophoresis
HPLC (SEC, IEC, RP, Affinity, etc.)
Protein Chips
SDS-PAGE
SDS PAGE Tools
Isoelectric Focusing (IEF)
Isoelectric Focusing
Separation of basis of pI, not Mw
Requires much higher voltages
Requires much longer period of time
IPG (Immobilized pH Gradient)
Typically done in strips or tubes (to facilitate 2D gel work)
Uses ampholytes to establish pH gradient
2D Gel Principles
