Molecular Biology Tools
Northern/Southern Blotting
Differential Display
RNAi (small RNA interference)
Serial Analysis of Gene Expression (SAGE)
DNA Microarrays or Gene Chips
Yeast two-hybrid analysis
Immuno-precipitation/pull-down
GFP Tagging & Microscopy
Yeast Protein Localization
Yeast Proteome Localized
Used 6234 yeast strains expressing full-length, chromosomally tagged green fluorescent protein (GFP) fusion proteins
Measured localization by fluorescence microscopy
Localized 75% of the yeast proteome, into 22 distinct subcellular localization categories
Provided localization information for 70% of previously unlocalized proteins
22 Different Cellular Zones
GFP Tagging the Yeast Proteome
Fluorescence Microscopy
Confirmation by Co-localization (GFP/RFP merging)
Results
