Bottlenecks
Producing enough protein for trials
Crystallization time and effort
Crystal quality, stability and size control
Finding isomorphous derivatives
Chain tracing & checking
Producing enough labeled protein for collection
Sample “conditioning”
Size of protein
Assignment process is slow and error prone
Measuring NOE’s is slow and error prone
Protein Expression
Robotic Crystallization
Synchrotron Light Source
MAD & X-ray Crystallography
High Throughput NMR
Automated Structure Generation
NMR & Structural Proteomics
NMR & Structural Proteomics
Auto-comparative Modeling
The Goal
