Relative instability
Identical ribosome binding sites in all vectors
Expression can be “leaky”
“Leaky” Expression Explained?
pQE Expression System
ADVANTAGES
T5 promoter
Native E. coli polymerase
IPTG inducible
Double lac operator following T5 promoter
Very tight regulation
DISADVANTAGES
Limited vector selection (~12)
Limited number of expression strains
M15[pREP4]
SG13009[pREP4]
pET vs. pQE expression of hLtn
Several different pET expression constructs of hLtn failed to yield any recombinant protein
Expression of hLtn in pQE30T
