参考文献：
[1]Wu C T, Morris J R. Genes, genetics and epigenetics: a correspondence [J]. Science, 2001, 293: 1103-1105.
[2]黄庆, 郭颖, 府伟灵. 人类表观基因组计划[J]. 生命的化学,2004, 24(2): 101-102.
[3]Dahl C, Guldberg P. DNA methylation analysis techniques [J]. Biogerontology, 2003, 4(4): 233-250.
[4]董玉玮,侯进慧,朱必才等. 表观遗传学的相关概念和研究进展[J]. 生命的化学, 2005, 22(1): 1-3.
[5]武立鹏, 朱卫国. DNA甲基化的生物学应用及检测方法进展[J]. 中国检验医学杂志, 2004, 27(7): 468-474.
[6]Riggs A D, Jones P D. 5-methylcytosine, gene regulation and cancer [J]. Adv Cancer Res, 1984, 40: 1-30.
[7]Bird A P. CpG-rich islands and the function of DNA methylation [J]. Nature, 1986, 321: 209-213.
[8]Cottrell S E. Molecular diagnostic applications of DNA methylation technology [J]. Clin Biochem, 2004, Jul, 37(7): 595-604.
[9]Jones P A, Baylin S B. The fundamental role of epigenetic events in cancer [J]. Nat Rev Genet, 2002, Jun. 3(6): 415-28.
[10]张永彪,褚嘉祐. 表观遗传学与人类疾病的研究进展 [J]. 遗传, 2005, 27(3): 466-472.
[11]Feinberg A P, Tycko B. The history of cancer epigenetic [J]. Nat Rev Cancer, 2004, 4(2): 143-153.
[12]黄琼晓, 金帆, 黄荷凤. DNA甲基化的研究方法学 [J]. 国外医学遗传学分册, 2004, 27(6): 354-358.
[13]Nuovo G J, Plaia T W, Belinsky S A, et al. In situ detection of the hypermethylation- induced inactivation of the p16 gene as an early event in oncogenesis [J]. Proc Natl Acad Sci USA, 1999, 96: 12754-12759.
[14]Soares J, Pinto A E, Cunha C V, et al. Global DNA hypomethylation in breast carcinoma: correlation with prognostic factors and tumor ptogression [J]. Cancer, 1999, 85: 112-118.
[15]Shinichi Toyooka, Nobuyoshi Shimizu. Models for studying DNA methylation in human cancer: a review of current status [J]. Drug discovery today: Disease Model, 2004, 1(1): 37-42.
[16]Cui H, Horon I L, Ohlsson R, Hamilton S R, Feinberg A P. Loss of imprinting in normal tissue of colorectal cancer patients with microsatellite instability [J]. Nat Med Nov, 1998, 4(11): 1276-80.
[17]Uhlmann K, Rohde K, Zeller C, et al. Distinct methylation profiles of glioma subtypes. [J] Int Cancer, 2003, Aug. 10, 106(1): 52-59.
[18]Kuo K C, McCune R A, Gehrke C W, et al. Quantitative reversed-phase high performance liquid chromatographic determination of major and modified deoxyribonucleosides in DNA [J]. Nucleic Acids Res, 1980, 8: 4763-4776.
[19]Fraga M F, Uriol E, Borja D L, et al. High-performance capillary electrophoretic method for the quantification of 5-methyl 2-deoxycytidine in genomic DNA: application to plant, animal and human cancer tissues [J]. Electrophoresis, 2002, 23: 1677-1681.
[20]Oefner, P J, Bonn G K, Huber C G, et al. Comparative study of capillary zone electrophoresis and high-performance liquid chromatography in the analysis of oligonucleotides and DNA. [J]. Chromatogr, 1992, 625(2): 331-3401.
[21]邓大君, 邓国仁, 吕有勇等. 变性高效液相色谱法检测CpG岛胞嘧啶甲基化 [J].中华医学杂志, 2001, 80(2), 158–1611.
[22]Wu J, Issa J, Hermen J, et al. Expression of an exogenous eukaryotic DNA methyl transferase gene induces transformation of NIN3T3 ceils [J]. Proc Natl Acad Sci USA, 1993, 90(19): 8891–8895.
[23]Oakeley E J, Podesta A, Jost J P. Developmental changes in DNA methylation of the two tobacco pollen nuclei during maturation [J]. Proc Natl Acad Sci USA, 1997, 94: 11721–11725.
[24]Oakeley E J, Schmitt F, Jost J P. Quantification of 5-methylcytosine in DNA by the chloroacetaldehyde reaction [J]. Biotechniques, 1999, 27: 744-6,748-50,752.
[25]Frommer M, McDonald L E, Millar D S, et al. A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands [J]. Proc Natl Acad Sci USA, 1992, 89: 1827–1831.
[26]朱燕. DNA的甲基化的分析与状态检测 [J]. 现代预防医学, 2005, 32(9): 1070-1073.
[27]沈佳尧, 侯鹏, 祭美菊等. DNA甲基化方法研究现状 [J]. 生命的化学, 2003, 23(2): 149-151.
[28]Herman J G, Graff J R, Myohanen S, et al. Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands [J]. Proc Natl Acad Sci USA, 1996, Sep 3. 93(18), 9821-9826.
[29]Kuppuswamy M N, Hoffmann J W, Kasper C K, Spitzer S G, Groce S L, Bajaj S P. Single nucleotide primer extension to detect genetic diseases: experimental application to hemophilia B (factor IX) and cystic fibrosis genes [J]. Proc Natl Acad Sci USA, 1991, 88: 1143–1147.
[30]Gonzalgo M L, Jones P A. Rapid quantitation of methylation differences at specific sites using methylation-sensitive single nucleotide primer extension (Ms-SNuPE) [J]. Nucleic Acids Res, 1997, 25: 2529–2531.
[31]Xiong Z, Laird P W COBRA: a sensitive and quantitative DNA methylation assay [J]. Nucleic Acids Res, 1997, 25: 2532–2534.
[32]Maekawa M, Sugano K, Kashiwabara H, et al. DNA methylation analysis using bisulfite treatment and PCR-single-strand conformation polymorphism in colorectal cancer showing microsatellite instability [J]. Biochem Biophys Res Commun, 1999, 262: 671–676.
[33]Cariello N F, Scott J K, Kat A G, et al. Resolution of a missense mutant in human genomic DNA by denaturing gradient gel electrophoresis and direct sequencing using in vitro DNA amplification: HPRT Munich [J]. Am J Hum Genet, 1988, May, 42(5): 726-34.
[34]Abrams E S, Stanton V P. Use of denaturing gradient gel electrophoresis to study conformational transitions in nucleic acids [J]. Methods Enzymol, 1992, 212: 71-104.
[35]Aggerholm A, Guldberg P, Hokland M, et al. Extensive intra- and interindividual heterogeneity of p15INK4B methylation in acute myeloid leukemia [J]. Cancer Res, 1999, 59: 436-441.
[36]黄庆, 府伟灵. DNA甲基化分析技术 [J]. 中国实验诊断学, 2005, 9(2): 304-306.
[37]Worm J, Aggerholm A, Guldberg P. In-tube DNA methylation profiling by fluorescence melting curve analysis [J]. Clin Chem, 2001, 47(7): 1183-9.
[38]Eads C A, Danenberg K D, Kawakami K, et al. MethyLight: a highthroughput assay to measure DNA methylation [J]. Nucleic Acids Res, 2000, 28: E32.
[39]范保星. DNA甲基化检测方法 [J]. 国外医学遗传学分册, 2002, 25(2): 99-101.
