Increased Photostability leading to High Sensitivity
Multiplexing capability
Increased Linearity resulting in Improved Quantifi cation of Protein Expression
Cost Savings compared to ECL
Compatibility with existing Imaging Systems
Enhanced chemiluminescence (ECL) is one of the most universally used blotting techniques with detection limits in the pico-gram range. The non radioactive nature of ECL makes it more attractive than radioisotope techniques which have similar sensitivity. However, even though ECL is fast and sensitive,it lacks the capability of detecting more than one antigen per blot.
EviFluor Western Blots allow for color multiplexing as well as a broad range of linearity for accurate quantifi cation. Now it is possible to observe the presence and amount of several antigens on the same blot and determine their concentrations, which translates to cost advantages over single color testing.
Western blot analysis using traditional organic fl uorophores have inherent limitations. Low photostability makes it difficult to image over long periods of time, resulting in lower sensitivity. Multiplexing is often difficult with organic dyes due to fact that each dye needs to be excited at different wavelengths requiring multiple excitation sources and complicating the design platform. In addition the broad emission spectra of the dyes can lead to cross talk between channels resulting in spectral overlapping.
