Threshold Cycle, C_T
 Correlates strongly with the starting copy number
 Is linear with the log of starting copy number

Practice with the animation so you understand the timing of this slide (if you haven’t used it before) - ask the audience to participate in this, it is a good way for you to determine the different comfort levels your audience has with the Ct concept.
See next slide for relationship between dilution series and threshold cycle values.

C_t值与模板起始浓度的关系
-模板起始浓度越高，C_t值越小
-C_t值与模板起始拷贝数的对数存在线性关系
如果模板浓度增加1倍，C_t值就提前1个循环到达
如果模板浓度减少1倍，C_t值就滞后1个循环到达

Mathematic Implications
Ideal PCR

1 C_T Difference = 2 fold difference in starting template amount
3.3 C_T Difference = 10 fold difference in starting template amount
Further elaboration on the exponential nature of PCR.  The mathematic expression that describes the process is shown.  Some basic implications are listed.

绝对定量可以得到某个样本中基因的拷贝数和浓度

Plot of Ct versus copy number.
Note the Dynamic Range of 10¹ to 10¹⁰.

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