The SYBR Green I is a double-stranded DNA (dsDNA) binding dye which is incorporated in the amplicon during the PCR. When SYBR Green I dye binds the Minor Groove of dsDNA, the intensity of fluorescence emission increases.
All the PCR products (including the non-specific ones) are detected in this way, but no probes are required.
3. Working scheme
The scheme below represents all the steps involved in the samples processing
Step1 and Step2: See Sample preparation in the page after logged in
Step 3: : The User must control if the genes they are looking for were already tested or not by the Service, searching in the “Is your gene already tested?” tool orthe “Target Genes Table". Then they have to contact the Service to choose the best strategy of working: the Service will provide to order the assay (see Assay selection in the page after logged in)
