20 mM dNTP stocks: Prepare from 100 mM stocks

80 ul 100 mM dNTP

40 ul 50:1 TE buffer

280 ul ddH₂O

400 ul

5 mM dNTP stocks: Prepare from 20 mM stocks

25 ul 20 mM dNTP

10 ul 50:1 TE buffer

65 ul ddH₂O

100 ul

2 mM dNTPs: 2 mM dATP, dCTP, dGTP, and dTTP in 5 mM Tris-HCl, pH 7.6, 0.1 mM EDTA

100 ul 20 mM dATP

100 ul 20 mM dCTP

100 ul 20 mM dGTP

100 ul 20 mM dTTP

100 ul 50:1 TE buffer

500 ul ddH₂O

1 ml

2 mM [alpha]-S-dNTPs: 2 mM [alpha]-S-dATP, [alpha]-S-dCTP, [alpha]-S-dGTP, and [alpha]-S-dTTP in 5 mM Tris-HCl, pH 7.6, 0.1 mM EDTA

100 ul 20 mM [alpha]-S-dATP

100 ul 20 mM [alpha]-S-dCTP

100 ul 20 mM [alpha]-S-dGTP

100 ul 20 mM [alpha]-S-dTTP

100 ul 50:1 TE buffer

500 ul ddH₂O

1 ml

3M NaCl (sodium chloride):

17.53 g NaCl

ddH₂O to 100 ml

10N NaOH (sodium hydroxide):

40 g NaOH

ddH₂O to 100 ml.

1N NaOH:

10 ml 10 N NaOH

ddH₂O to 100 ml

9.5M NH4OAc (ammonium acetate):

73.23 g NH4OAc

ddH₂O to 100 ml

8.0M NH4OAc:

61.69 g NH4OAc

ddH₂O to 100 ml

上一页  [1] [2] [3] [4] [5] [6] [7] [8] [9] [10]  ... 下一页  >>