20.33 g MgCl2-6H₂O

ddH₂O to 100 ml

1 M MgSO4 (magnesium sulfate):

12.04 g MgSO4

ddH₂O to 100 ml (autoclave)

1 M MnCl2 (manganese chloride):

1.98 g MnCl2 (Sigma M-8530)

ddH₂O to 10 ml (store protected from light)

1 M MOPS:

20.93 g MOPS (Sigma M-1254)

Dissolve in 80 ml ddH₂O, adjust pH to 7.5 with 1 N NaOH, and bring volume to 100 ml.

10X MOPS buffer: 400 mM MOPS, pH 7.5, 500 mM NaCl, 100 mM MgCl2 in double distilled water.

400 ul 1 M MOPS, pH 7.5

170 ul 3 M NaCl

100 ul 1 M MgCl2

330 ul ddH₂O

1 ml

2.7 M MOPS (acid form):

5.65 g MOPS (acid form)

ddH₂O to 10 ml

MOPS-Acid buffer: 1.35 M MOPS (acid form), 100 mM MgCl2 in double distilled water.

500 ul 2.7 M MOPS (acid form)

100 ul 1 M MgCl2

400 ul ddH₂O

1 ml

10X Mn2+/isocitrate buffer: 50 mM MnCl2, 150 mM isocitrate (Na salt), 25% glycerol in double distilled water

50 ul 1 M MnCl2

150 ul 1 M isocitrate

250 ul glycerol

550 ul ddH₂O

1 ml

10x MTBE (Modified Tris-borate-EDTA buffer): 1.3 M Tris, 0.4 M Boric acid, and 0.02 M EDTA in double distilled water.

162 g Tris base

27.5 g Boric acid

9.3 g Na2EDTA

ddH₂O to 1 L

Nucleotide ordering information:

100 mM dATP 27-2050-01 Pharmacia

100 mM dCTP 27-2060-01 Pharmacia

100 mM dGTP 27-2070-01 Pharmacia

10 mM c7dGTP 988 537 Boehringer-Mannheim

100 mM dTTP 27-2080-01 Pharmacia

5 mM ddATP 27-2057-00 Pharmacia

5 mM ddCTP 27-2065-00 Pharmacia

5 mM ddGTP 27-2075-00 Pharmacia

5 mM ddTTP 27-2085-00 Pharmacia

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