5X Taq "A" termination mix: 62.5 uM dATP, 250 uM dCTP, 375 uM c7dGTP, 250 uM dTTP and 1.5 mM ddATP in 5 mM Tris-HCl, pH 7.6 and 0.1 mM EDTA.

20 ul 20 mM dATP

80 ul 20 mM dCTP

240 ul 10 mM 7deaza-dGTP

80 ul 20 mM dTTP

1920 ul 5 mM ddATP

640 ul 50:1 TE buffer

3420 ul sddH₂O

6.4 ml

5X Taq "C" termination mix: 250 uM dATP, 62.5 uM dCTP, 375 uM c7dGTP, 250 uM dTTP and 0.75 mM ddATP in 5 mM Tris-HCl, pH 7.6 and 0.1 mM EDTA.

80 ul 20 mM dATP

20 ul 20 mM dCTP

240 ul 10 mM 7deaza-dGTP

80 ul 20 mM dTTP

960 ul 5 mM ddCTP

640 ul 50:1 TE buffer

4380 ul sddH₂O

6.4 ml

5X Taq "G" termination mix: 250 uM dATP, 250 uM dCTP, 94 uM c7dGTP, 250 uM dTTP and 0.125 mM ddGTP in 5 mM Tris-HCl, pH 7.6 and 0.1 mM EDTA.

160 ul 20 mM dATP

160 ul 20 mM dCTP

120 ul 10 mM 7deaza-dGTP

160 ul 20 mM dTTP

320 ul 5 mM ddGTP

1280 ul 50:1 TE buffer

10600 ul sddH₂O

12.8 ml

5X Taq "T" termination mix: 250 uM dATP, 250 uM dCTP, 375 uM c7dGTP, 62.5 uM dTTP and 1.25 mM ddTTP in 5 mM Tris-HCl, pH 7.6 and 0.1 mM EDTA.

160 ul 20 mM dATP

160 ul 20 mM dCTP

480 ul 10 mM 7deaza-dGTP

40 ul 20 mM dTTP

3200 ul 5 mM ddTTP

1280 ul 50:1 TE buffer

7480 ul sddH₂O

12.8 ml

20X TAE buffer: 0.8 M Tris, 0.4 M NaOAc, and 0.04 M Na2EDTA, and glacial acetic acid to pH 8.3 in double distilled water.

96.9 g Tris base

32.8 g NaOAc-3H₂O

14.9 g Na2EDTA

Dissolve in approx. 700 ml of double distilled water, adjust the pH to 8.3 with glacial acetic acid, and bring to 1 L with ddH₂O.

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