Seeds of Arabidopsis were sown onto F2 compost (Levington Horticulture, Ipswich, UK) in shallow trays consisting of individual 4- × 4-cm pots (P40, Cookson Plantpak, Maldon, UK), with several seeds per pot. Compost for short-day-grown plants was treated with systemic insecticide (Intercept 70WG, Levington Horticulture) before sowing. After 2 to 5 d of cold treatment at 4°C, trays were transferred to 21°C, 8-h (short) or 16-h (long) photoperiods and watered with tap water. Light intensities were 130 and 50 µmol m 2 s 1 for the short and long photoperiod, respectively. Seedlings were thinned out to one per pot after germination.
温室


培养室
[1] [2] [3] [4] 下一页