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拟南芥的培养和管理
作者:Bobby 来源:Bobby博客 时间:2007-7-21
    Seeds of Arabidopsis were sown onto F2 compost (Levington Horticulture, Ipswich, UK) in shallow trays consisting of individual 4- × 4-cm pots (P40, Cookson Plantpak, Maldon, UK), with several seeds per pot. Compost for short-day-grown plants was treated with systemic insecticide (Intercept 70WG, Levington Horticulture) before sowing. After 2 to 5 d of cold treatment at 4°C, trays were transferred to 21°C, 8-h (short) or 16-h (long) photoperiods and watered with tap water. Light intensities were 130 and 50 µmol m 2 s 1 for the short and long photoperiod, respectively. Seedlings were thinned out to one per pot after germination.

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