Data Normalization
Normalizing measured values for sample load is essential for any quantiative technique
Methods used for qRT-PCR:
Total RNA-A260:
Very convenient but not specific ribogreen: sensitive, fairly specific, costly 18SrRNA qPCR: the Cadillac, cost
Housekeeping Transcripts-b-Actin: works for most projects, not all
Cyclophilin & 36B4: works well if protein synthesis is not elevated
18SrRNA Testing for Pol II Transcripts
Samples from formaldehyde fixed and embedded human heart tissue pre- and post-LVAD implantation.
Acknowledgements
Peter J.A. Davies, M.D., Ph.D.
David Loose-Mitchell, Ph.D.
George M. Stancel, Ph.D.
Xiaoying Wang
Nancy Shipley
