Thermocycler Settings
Applied Biosystems SDS software defaults to one thermocycler setting to match the contents of their kits
Standard cycle is: 50°C-2 min; 95°C-10 min; 40 cycles of 95°C-15 sec; 60°C-60 sec = a full 2 hr run time
Assumes use of Amperase-like enzyme and hotstart Taq
Amperase and hotstart Taq are not necessary for RT-PCR
Can cut cycle time down to 95°C-1 min and 40 cycles of 95°C-12 sec; 60°C-60 sec = 1 hr 36 min run time
At 95°C- 12 sec; 60°C-30 sec = 1 hr 15 min run time
At 94°C-12 sec; 60°C-20 sec = 1 hr 05 min
Setting the Baseline & Threshold Pre-adjustment
Setting the Baseline & Threshold Post-adjustment
