1.Materials
1.1.Probe Preparation
1.1.1.Cell Inoculation
1.96-well, deep square-well round bottom plate (E&K Scientific Ritter Riplate).
2.Super Broth (SB: 10 gm 4-Morpholinepropanesulfonic acid (MOPS), 20 gm Bacto Yeast Extract, 30 gm Tryptone Peptone/L).
3.Antibiotic (80 µg/ml final concentration Carbenicillin- for pFLC-I orpBS SK, 80 µg/ml chloramphenicol for pOT2 or pOTB7).
4.Multichannelpipette (Brand).
5.Multichannel pipette (Qiagen Multi Electrapette 1550).
6.Airpore Tape Sheets (Qiagen).
7.Speedball roller (E&K Scientific).
8.Incubator/Shaker (Innova 4300).

1.1.2. PCR
1. 96-well semi-skirt PCR plate (E&K Scientific).
2. Deoxynucleotides (Promega, store at -20℃).
3. Sequence specific primers ( Invitrogen, store at -20℃).
pFLC-I vector:
M13 (-21) 5’ TGTAAAACGACGGCCAGT 3’
M13 (REV) 5’ GGAAACAGCTATGACCATG 3’
p0T2a vector:

4. DNA Polymerase (DyNAzyme EXT DNA Polymerase-MJ Research, store at -20℃).
5. Inoculated cells (can be stored for up to 7 days at 4 ℃).
6. Multichannel pipette (Brand).
7. GeneAmp PCR System 9700 (Applied Biosystems).
8. Speedball roller (E&K Scientific).
9. Aluminum plate sealers (Polarseal:Foil adhesive Tape for multiwell plates T592100) (E&K Scientific) or 8-strip PCR caps (E&K Scientific).

1.1.3. PCR Product Purification (G-50 column chromatography)
1. Sephadex ™G-50 Superfine (Amersham Pharmacia Biotech).
2. MultiScreen column loader-45 µl (Milliore) MAHV N45 filter plate (Millipore).
3. MultiScreen Centrifuge Alignment Frame (Millipore).
4. Multichannel pipette (Brand).
5. Sterile 0.1% diethyl pyrocarbonate (DEPC, Sigma, handle carefully, toxic) treated H₂O(autoclaved).
6. Eppendorf Centrifuge 5810 (equipped with 96-well plate holders).
7. 96-well semi-skirt PCR plate (E&K Scientific).
9. Aluminum plate sealers or 8-strip PCR caps (E&K Scientific).

1.1.4. RNA Probe Preparation
1. 96-well semi-skirt PCR plate (E&K Scientific).
2. Multichannel pipette (Brand).
3. Speedball roller (E&K Scientific).
4. GeneAmp PCR System 9700 (Applied Biosystems).
5. Aluminum plate sealers or 8-strip PCR caps (E&K Scientific).
6. Eppendorf Centrifuge 5810 (equipped with 96-well plate holders).
7. 2X Polymerase mix (2U T7 RNA polymerase (Roche, store at -20℃), 4.6U RNase inhibitor (Amersham Parmacia Biotech), 10mM NTPs (Roche, store at -20℃), 3.5mM digoxigenin-11-UTP (Roche, store at -20℃), 40mM Tris pH 8.0, 6mM MgCl₂, 10mM DTT and 2mM spermidine).

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