A heating plate is placed against the gel to ensure a constant performing temperature.
Gel buffer is loaded into the top tank…
…and into the bottom tank.
A final look, then the door is shut and the program begun.
In summary
! PCR is a simple technique to obtain many copies of specific DNA fragments
! Three steps are involved in PCR: denaturation,annealing and extension
! To ensure success, care should be taken both in preparing the reaction mixture and setting up the cycling conditions
