dNTPs. The concentration of each dNTP (dATP, dCTP, dGTP, dTTP) in the reaction mixture is usually 200 μM. These concentrations must be checked as being equal,because inaccuracies will increase the degree of misincorporation.
PCR procedures: equipment
! Micropipettes
! Thermocycler
! Electrophoresis units
! Power supply units
! Photographic equipment
PCR technology in pictures
The following photographs demonstrate how PCR technology is carried out
The PCR mixture is prepared on ice. Safety clothing is not required, but may benefit the reaction unless good sterile techniques are practised.
The PCR reactions are loaded into the thermocycler.
