Select Next file button to view the second chromatogram. When you are done exit the Trev program to signal to the Pregap to continue.

When the pregap finishes processing and assembling sequences the Textual Output will display ***   Processing finished   *** message. 

Exit the Pregap

To view the assembled contig file launch the Gap4 program from the Start Menu.
Open the generated database by selecting File -> Open menu. 
Browse to the directory you created and select the file nameofdatabase.0.aux 
Right click on the graphical preview of the contig sequence in the Contig Selector window and select Edit contig.


The contig editor will display three sequences: two sequences obtained from chromatograms and the contig sequence. To see the chromatogram for a particular position double click the nucleotide. 
Explore the different menus and display options. 
Edit the contig sequence if required. 
When you are done select File Save consensus form the GAP window.

If you require to save portion of your contig file select single from the Save consensus window. Modify the size of the contig sequence, add “.txt” extension to the name of the contig file to make it more convenient to open and save. The sequence file will be saved in FASTA format. A sequence in FASTA format begins with a greater-than symbol (>), followed by a single-line description and then (starting a new line) lines of sequence data.

上一页  [1] [2] [3] [4] [5] 下一页