31. Yeast Sporulation Medium (酵母菌产生子囊孢子培养基)
(1) Kleyn Medium
KH₂PO₄ 0.012% K₂HPO₄ 0.02%
Sodium acetate (醋酸钠) 0.5% Glucose (葡萄糖) 0.062%
NaCl 0.062% Peptone (蛋白胨) 0.25%
Biotin (生物素) 2μg% Agar (琼脂) 2%
Trace element solution (混合盐类) 1ml/100ml
[Note]: Trace element solution （混合盐类溶液）： MgSO₄.7H₂O 0.4%, NaCl 0.4%, CuSO₄.5H₂O 0.002%, MnSO₄.4H₂O 0.2%, FeSO₄.4H₂O 0.2%
(2) Gorodkowa Medium
Glucose (葡萄糖) 0.1% Peptone (蛋白胨) 1%
NaCl 0.5% Agar (琼脂) 2%
(3) McClary Medium
Glucose (葡萄糖) 0.1% KCl 0.18%
Yeast juice (酵母汁) 0.25% Sodium actate (醋酸钠) 0.82%
Agar (琼脂) 1.5%
32. BPY Medium (BPY 培养基)
Beef extract (牛肉膏) 5g Prptone (蛋白胨) 10g
NaCl 5g Yeast extract (酵母膏) 5g
Glucose (葡萄糖) 5g Agar (琼脂) 1-2%
Distilled (蒸馏水) 1000ml pH 7.0
适用范围：大肠埃希氏菌（大肠杆菌）、恶臭假单胞菌
33. LB Medium (LB 培养基)
Yeast extact (酵母膏) 5g Peptone (蛋白胨) 10g
NaCl 10g Agar (琼脂) 1-2%
Distilled water (蒸馏水) 1000ml pH 7.0
适用范围：大肠埃希氏菌（大肠杆菌）
34. Penssay Broth (Penssay 肉汤)
Beef extract (牛肉膏) 1.5g Yeast extract (酵母膏) 1.5g
Tryptone (胰蛋白胨) 5g NaCl 3.5g
Glucose (葡萄糖) 1g K₂HPO₄.3H₂O 4.8g
Distilled water (蒸馏水) 1000ml pH 7.2
35. Blond Agar (血琼脂培养基)
Peptone (蛋白胨) 10g Glucose (葡萄糖) 2g
NaCl 5g NaH2PO4 2.5g
Sodium citrate (柠檬酸钠) 5g Brain infusion (脑浸汁) 800ml
Pig heart infusion (猪心浸) 200ml Sleep Blood (羊血) 10%
pH 7.2
[Note]：Preparation of pig brain and pig heart infusion：
（1） Smash up 100g fresh pig heart （without fat and blood vessel）.Add distilled water up to 1000ml;
（2） Cut 200g fresh pig brain (without blood vessel). Add distilled water up to 1000ml;
（3） Keep the two infusions at 50℃ for 1 hr , then boil for 15 minutes;
（4） Cool and filtrate through gauze . [注]猪心和猪脑浸汁法：（1）新鲜猪心除去心头和脂肪，搅碎100克加蒸馏水至1000毫升；（2）新鲜猪脑除去血管，取200克加蒸馏水至1000毫升；（3）50℃保温1小时，然后煮开15分钟；（4）冷却后用纱布过滤备用。
36. Cellulose﹣decomposing Bacteria Synthetic Medium (纤维细菌合成培养基)
NaCl 6g MgSO₄.7H₂O 0.1g
KH₂PO₄ 0.5g CaCl₂ 0.1g
K₂HPO₄ 2.0g Yeast extract (酵母膏) 1.0g
(NH₄)₂SO₄ 2.0g Distilled water (蒸馏水) 1000ml
Filter paper strip: one strip (滤纸条：一片) pH 7.0-7.4
[Note]: So;id medium for cellulose-decomposing bacteria: Carboxymethyl Cellulose 2% Agar 2%. （斜面培养基去滤纸条用2%琼脂及2%羧甲基纤维素钠 CMCNa ）
37. Clostridium Pasreurianum Synthetic Medium （巴氏梭状芽孢杆菌合成培养基）
Glucose （葡萄糖） 1% MnSO₄ 0.001%
KH₂PO₄ 0.05% FeSO4 0.001%
K₂HPO₄ 0.05% Yeast extract（酵母膏） 0.1%
MgSO₄.7H₂O 0.02% Peptone (蛋白胨) 0.01%
NaCl 0.001% CaCO₃ 0.5%
Distille water (蒸馏水) 100% pH 7.0

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