(2)0.05mol/L Tris-HCl缓冲液（pH7.19～9.10）
pH0.2mol/L Tris(ml)0.2mol/L HCl(ml)H2O
7.1910.018.012.0
7.3610.017.013.0
7.5410.016.014.0
7.6610.014.015.0
7.7710.014.016.0
7.8710.013.017.0
7.9610.012.018.0
8.0510.011.019.0
8.1410.010.020.0
8.2310.09.021.0
8.3210.08.022.0
8.4110.07.023.0
8.5110.06.024.0
8.6210.05.025.0
8.7410.04.026.0
8.9210.03.027.0
9.1010.02.028.0

　　(3)0.2mol/L醋酸盐缓冲液（pH3.6～5.6）
pH0.2mol/L醋酸（ml）0.2mol/L醋酸钠（ml）
3.646.33.7
3.844.06.0
4.041.09.0
4.236.813.2
4.430.519.5
4.625.524.5
4.820.030.0
5.014.835.2
5.210.539.5
5.48.841.2
5.64.845.2

　　(4)0.1mol/L二甲胂酸钠缓冲液（pH5.0～7.4）
pH0.2mol/L二甲胂酸钠（ml）0.2N HCl(ml)蒸馏水
5.02523.551.5
5.22522.552.5
5.42521.553.5
5.62519.655.5
5.82517.457.5
6.02514.860.3
6.22511.963.1
6.4259.265.8
6.6256.768.3
6.8254.770.3
7.0253.271.8
7.2252.172.9
7.4251.473.6

　　注：HCI可由NCO3代替，配制成二甲胂酸钠-HNO3缓冲液。
　　（5）0.2mol/L碳酸盐缓冲液（pH9.2～10.7）
pH0.2mol/L Na2CO3(ml)0.2mol/L NaHCO3(ml)
9.24.046.0
9.37.542.5
9.49.540.5
9.513.037.0
9.616.034.0
9.719.530.5
9.822.028.0
9.925.025.0
10.027.522.5
10.130.020.0
10.233.017.0
10.335.514.5
10.438.511.5
10.540.59.5
10.642.57.5
10.745.05.0

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