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RNA analysis is the starting point for many molecular biology procedures. The RNA Analysis Notebook provides an introduction to RNA procedures such as……
The isolation and purification of DNA is crucial to many applications in molecular biology and techonogy. Over the past decade, the purification of DN……
Cloning Enzymes, one in the Enzyme Resource Guide series, highlights those enzymes important in nucleic acid cloning procedures. Enzymes that modify n……
Introduction You may wish to subclone your PCR product into a plasmid cloning vector. When PCR was in its infancy, researchers found that subcloning P……
Subcloning is a basic molecular biology procedure used to move inserts from one vector to another. Essentially all subcloning reactions proceed as fol……
最新分子生物学实验技术……
双向电泳的技术流程和样品制备史须北京大学人类疾病基因研究中心基因组学与蛋白质组学基因组学:蛋白质组学:可视为分子生物学的大规模筛选技术,目的在于归类细胞中的蛋白质的整体分布,鉴定并分析感兴趣的个别蛋白,最终阐明它们的关系与功能。上述两种学科的研究内容在互补的水平上研究了细胞的分子架构,又都在各自的……
如何选择凝胶?五大类层析方法 - 1. 凝胶过滤 (GC) 2. 离子交换 (IEX) 3. 亲和层析 (AC) 4. 疏水层析 (HIC)  ……
DNA序列测定 即核酸DNA分子一级结构的测定,是现代分子生物学一项重要的技术。 1963年,Sanger和Thompson等人第一次完成胰岛素51个氨基酸的……
试剂配制(一)母液1.0mol/L Tris·HCl Tris (MW121.14) 30.29g蒸馏水 200ml溶解后,用浓盐酸调pH至所需点(见下所示),最后用蒸馏水定容至250ml,高温灭菌后室温下保存。 PH HCl7.4 约17ml7.5 约16m7.6 约15ml8.0 约10ml1.……


