Introduction to RNAi and siRNAs 3
Inducing RNAi in Mammalian Cultured Cells: Experimental Overview.5
Comparing siRNA Synthesis Methods: Choose the Best Approach6
siRNA Decision Tree: A Guide to Designing siRNA Experiments.. 7
siRNA Design: Ambion Answers Your Questions. 8
How to Order siRNAs9
siRNA Delivery: Ambion Answers Your Questions10
Getting the Most from Your RNAi Data: Which Controls to Use.12
Elucidating Gene Function with RNAi Screens..14
siRNAs, siRNA Synthesis, and siRNA Libraries
Silencer® Pre-designed siRNAs..16
Silencer® Validated siRNAs.17
Custom siRNA Design18
Custom siRNA Synthesis. .18
Silencer® siRNA Libraries..19
Silencer® CellReady siRNA Libraries.. .20
siRNA Controls and Assessing siRNA Delivery Efficiency
Silencer® CellReady siRNA Transfection Optimization Kit.. .21
Silencer® siRNA Controls..22
KDalert™ GAPDH Assay Kit23
Silencer® Labeled Control siRNAs .24
Silencer® siRNA Labeling Kits.. .24
Antibodies Matched to Silencer® Validated siRNAs and Silencer® Control siRNAs. .25
siRNA Synthesis by in Vitro Transcription
Silencer® siRNA Construction Kit .26
Silencer® siRNA Cocktail Kits27
siRNA Delivery
siPORT™ NeoFX™ Transfection Agent.28
siPORT™ Amine Transfection Agent..29
siPORT™ Lipid Transfection Agent29
Silencer® siRNA Transfection II Kit29
siPORT™ Electroporation Kit and Buffer.. .30
siPORTer™-96 Electroporation Chamber.. .31
siRNA Expression Vectors and Related Products
pSilencer™ siRNA Expression Vectors. .32
siPORT™ XP-1 Transfection Agent33
pSilencer™ 5.1 Retro System .34
pSilencer™ adeno 1.0-CMV System.. .35
Reagents for RNAi in Non-mammalian Systems
MEGAscript® RNAi Kit.. .36
Silencer® Drosophila RNAi Library37
MEGAscript® High Yield Transcription Kits.37
Bulk reagents, Enzymes, and Kits .37
Kits and Reagents for Assessing the RNAi Effect
Applied Biosystems’ TaqMan® Gene Expression Assays .38
Cells-to-Signal™ Kit..38
RNAqueous®-4PCR Kit.. .38
PARIS™ (Protein and RNA Isolation System) Kit. .38
Working with RNA
RNaseZap® and RNaseZap® Wipes.. .39
RNase-free Tips and Tubes .39
Maintaining an RNase-free Lab.39
RNAi Information Resources
Technical Seminars..40
Technical Support from Highly Trained Scientists .40
Silencer e-Newsletter.40
TechNotes Newsletter40
Online RNA Interference Resource..41
Online siRNA Delivery Resource .41
Appendix: siRNA Delivery Conditions by Cell Type
Optimized Transfection Conditions for Various Cell Types. .42
Optimized Conditions for Single Cuvette Electroporation
with siPORT™ Electroporation Buffer.43
Optimized Conditions for Electroporation with the siPORTer™-96 Electroporation Chamber and siPORT™ Electroporation Buffer..43
