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Electrophoresis: the movement of ions in an electric field.
Electrophoretic techniques exploit the fact that different ions have different mobilities in an electric field and so can be separated by electrophoresis.
Properties of proteins
• Hydrophobicity
;Solubility, location, globularity etc.
• UV absorbity
; depends on the Tyr and Trp content
• Isoelectric point (PI)
; pH at which a molecule has no net charge.
• Molecular weight (MW)
; SDS PAGE, protein sequencing