Contents
Preface V
Color Plates IX
Part I. Introduction
Chapter 1: Overview
M. Tomita 1
Part II. Analytical Methods for Metabolome Sciences
Chapter 2: Development and Application of Capillary
Electrophoresis-Mass Spectrometry Methods to Metabolomics
T. Soga 7
Chapter 3: Application of Electrospray Ionization Mass Spectrometry
for Metabolomics
R. Taguchi 25
Chapter 4: High-Performance Liquid Chromatography and Liquid
Chromatography/Mass Spectrometry Analyses of Metabolites in
Microorganisms
H. Miyano 37
Chapter 5: Metabolome Profiling of Human Urine with Capillary Gas
Chromatography/Mass Spectrometry
T.Kuhara 53
Chapter 6: Metabolic Profiling by Fourier-Transform Ion Cyclotron
Resonance Mass Spectrometry (FT-ICR-MS) and Electrospray
Ionization Quadrupole Time-Of-Flight Mass Spectrometry
(ESI-Q-TOF-MS)
K. Hirayama 75
Chapter 7: Metabolome Analysis by Capillary Electrophoresis
L. Jiaand S. Terabe 91
Chapter 8: High-Performance Liquid Chromatography for
Metabolomics: High-Efficiency Separations Utilizing Monolithic
Silica Columns
T. Ikegami, H. Kobayashi, H. Kimura, V.V. Tolstikov, O. Fiehn, and N. Tanaka 107
Part III. Applications of Metabolome Analysis to Biosciences
Chapter 9: Combined Analysis of Metabolome and Transcriptome:
Catabolism in Bacillus subtilis
T. Nishioka, K. Matsuda, and Y. Fujita 127
Chapter 10: Metabolomics in Arabidopsis thaliana
K. Saito 141
Chapter 11: Lipidomics: Metabolic Analysis of Phospholipids
R. Taguchi 155
Chapter 12: Chemical Diagnosis of Inborn Errors of Metabolism and
Metabolome Analysis of Urine by Capillary Gas
Chromatography/Mass Spectrometry
T.Kuhara 167
Part IV. Metabolome Informatics
Chapter 13: Introduction to the ARM Database: Database on Chemical
Transformations in Metabolism for Tracing Pathways
M.Arita 193
Chapter 14: The Genome-Based E-CELL Modeling (GEM) System
K. Arakawa, Y. Yamada, K. Shinoda, Y. Nakayama, and
M.Tomita 211
Chapter 15: Hybrid Dynamic/Static Method for Large-Scale
Simulation of Metabolism and its Implementation to the E-CELL
System
Y. Nakayama 221
Part V. Metabolomics and Medical Sciences
Chapter 16: Metabolomics and Medical Sciences
T. Nishioka 233
Index 245