MANUAL EDITORS . 4
INTRODUCTION .. 6
LABORATORY PROCEDURES
Biosafety.. 8
Specimen Codes .. 10
Specimen Processing  13
Specimen Storage Recommendations ... 23
Specimen Labeling ... 25
Specimen Shipment .. 26
Cryopreservation and Thawing of PBMC . 33
Pipette Calibration Procedures (gravimetric; colorimetric)  35
ASSAY PROTOCOLS
PHA-Stimulated Uninfected Donor PMBC... 41
Qualitative PBMC Macrococulture Assay. 46
Qualitative PBMC Micrococulture Assay . 53
Quantitative PBMC Micrococulture Assay ... 61
Quantitative Plasma HIV Culture . 67
Syncytium-Inducing (MT-2) Assay... 73
HIV Drug Susceptibility Assays 78
Drug Resistance Screening Assay (NEN RT-Detect™)  92
Cytomegalovirus Drug Susceptibility Assay.. 106
HIV DNA PCR 117
Chiron Quantiplex® (bDNA) HIV RNA 2.0 Assay... 126
Roche Amplicor HIV Monitor™ (RT PCR) Assay ... 138
Organon Technika NASBA™ HIV-1 RNA QT Assay.. 148
Codon 215 Mutation PCR Detection for HIV DNA . 165
Codon 215 Mutation PCR Detection for HIV RNA.. 172
Neutralizing Antibody - Infectivity Reduction Assay. 182
HIV-1 p24 Ag ELISA (direct) - Dupont... 187
HIV- 1 p24 Ag ELISA (immune complex dissociation) - Dupont . 193
HIV-1 p24 Ag Microelisa - Organon Teknika... 200
HIV-1 p24 Ag Assay, (direct, murine monoclonal) - Coulter  206
HIV-1 p24 Ag Assay (immune complex dissociation, monoclonal) - Coulter. 213
HIV-1 p24 Ag Assay (direct, monoclonal) - Abbott.. 221
HIV-1 p24 Ag Assay (immune complex dissociation, monoclonal) - Abbott . 227
HIV-1 p24 Ag Assay (culture supernatant, monoclonal) - Abbott . 234
HIV-1 p24 Ag Assay (direct, polyclonal) - Abbott 240
HIV-1 p24 Ag Assay (immune complex dissociation, polyclonal) - Abbott ... 246
HIV-1 p24 Ag Assay (culture supernatant, polyclonal) - Abbott ... 253