凝胶电泳是每个做分子生物学天天都要打交道的基本技术。电泳之后的信息处理与电泳本身同样重要。目前有大量软件可以用于分析电泳结果,比较有名的比如BandScan、BandLeader、Sigma Gel等等。今天要向大家介绍的是来自Bio-Rad的1D凝胶定量软件Quantity One(Bio-Rad还有一个做2D凝胶分析的软件PDQuest)。
共享版可试用30天,30天后依然能处理,只是不能保存和打印。
Overview of Quantity One
Quantity One is a powerful, flexible software package for imaging and analyzing 1-D electrophoresis gels, dot blots, arrays, and colonies.
The software is supported on Windows and Macintosh operating systems and has a graphical interface with standard pull-down menus, toolbars, and keyboard commands.
Quantity One can image and analyze a wide variety of biological data, including radioactive, chemiluminescent, fluorescent, and color-stained samples acquired from densitometers, phosphor imagers, fluorescent imagers, and gel documentation systems.
An image of a sample is captured using the controls in the imaging device window and displayed on your computer monitor. Image processing and analysis operations are performed using commands from the menus and toolbars.
Images can be magnified, annotated, rotated, and resized. They can be printed using standard and video printers.
All data in the image can be quickly and accurately quantitated using the Volume tools.
The lane-based functions can be used to determine molecular weights, isoelectric points, VNTRs, presence/absence and up/down regulation of bands, and other values. The software can measure total and average quantities, determine relative and actual amounts of protein, and count colonies in a Petri dish.
The software can cope with distortions in the shape of lanes and bands. Lanes can be adjusted along their lengths to compensate for any curvature or smiling of gels.
Image files can be shared among all The Discovery Series™ software. Images can also be easily converted into TIFF format for compatibility with other Macintosh and Windows applications.




