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摘要:系统研究了吸附剂(活性炭、PVP)与柠檬酸、半胱氨酸、谷胱甘肽、维生素C和硫代硫酸钠5种抗氧化剂对蝴蝶兰组培中褐化的影响。结果表明:活性炭能有效控制褐化和促进生长,但不利于分化;谷胱甘肽控制褐化的效果虽不及活性炭,但综合效果最佳。
关键词:蝴蝶兰;组织培养;褐化;褐化控制
Studies of Antibrowning in the Tissue Culture of Phalaenopsis
Liu Zhenhua。一
,
Ge Hong ,Guo Shaoxia ,Liu Hongtao’,
Cao Canjing ,Zhou Yujie。,and Li Qiuxiang。
(’Institute of Vegetables and Flowe~,Chinese Academy ofAgricultural Sciences,Bering 100081,China; The Landscape Architecture
and Art Department,Laiyang Agricultural College,Laiyang 265200,China) ’
Abstract:In order to control the browning of tissue culture on Phalaenopsis,the contrast test of adsorbent:activated charcoal,PVP,and five antioxidants:citric acid,cysteine,glutathione,vitamin C,sodium thiosulfate in tissue culture of Phalaenopsis were studied.The results showed that activated charcoal Can control browning and promote growth.But at the same time,it Was adverse to the diferentiation.Although the glutathione,which the effect of antibrowning Was not as good as activated charcoal’S,its resultant effect Was the best one.
Key words:Phalaenopsis spp.;Tissue cuhure; Browning; Antibrowning