关键词：RNAi；药物靶点；高通量筛选；RNAi药物；多药耐药性
Development and applications of RNAi technology in drug research
Wang Lina, Yuan Chonggang*
(College of Life Science, East China Normal University, Shanghai 200062, China)
Abstract: RNA interference (RNAi) is a process in which double-stranded RNA (dsRNA) induces sequencespecific posttranscriptional gene silencing of homologous transcripts. In mammalian cells, RNAi can be triggered by 21-25 nucleotide duplexes of small interfering RNA (siRNA). In the postgenome era, RNAi has broad application prospect in probing gene function and drug research. This review summarises development and applications of RNAi technology in drug research，such as drug-target discovery and identification, RNAi reagent, multidrug resistance over the past few years.
Key words: RNA interference; drug target; high throughput screening; RNAi reagent; multidrug resistance
