Contents
List of Contributors viii
Foreword xvi
Chapter1 DNA Polymorphism and Taxonomy of Brucella Species
Axel Cloeckaert and Nieves Vizcaino 1
Chapter2
Molecular Diagnostics of Animal Brucellosis: A Review of PCRBased
Assays and Approaches
Betsy J.Bricker
24
Chapter3
Polymerase Chain Reaction: A Powerful New Approach for the
Diagnosis of Human Brucellosis
Juan D.Colmenero, María I.Queipo-Ortuño and Pilar Morata
51
Chapter4
A Brucella melitensis Genomic Database
Xavier De Bolle, Christophe Lambert, Eric Depiereux and Jean-
Jacques Letesson
67
Chapter5
Comparative Genomics of Brucella melitensis, B. suis, and B.
abortus
Shirley M.Halling, Cynthia Gibas and Stephen Boyle
84
Chapter6 Comparative Proteomics of Brucella Species
Vito G.DelVecchio and Cesar V.Mujer 102
Chapter7 Brucella Virulence: A Matter of Control
Jean-Jaques Letesson and Xavier De Bolle 113
Chapter8
Brucella Lipopolysaccharide: Structure, Biosynthesis and Genetics
Maite Iriarte, David González, Rose M.Delrue, Daniel Monreal,
Raquel Conde, Ignacio López-Goñi, Jean-Jacques Letesson, and
Ignacio Moriyón
152
Chapter9
Brucella Cyclic β-1,2-Glucans: Structure, Biosynthesis, Biological
Activities and Role in Virulence
Nora Iñón de Iannino and Rodolfo A.Ugalde
184
Chapter10
The Brucella BvrS/BvrR and Related Two-Component Regulatory
Systems of the α-2 Proteobacteria: Common Regulatory Strategies of
Animal and Plant Pathogens and Endosymbionts
Ignacio López-Goñi, Lorea Manterola and Shen Q.Pan
204
Chapter11 Erythritol Metabolism and Virulence in Brucella
Juan M.García-Lobo and Felix J.Sangari García 223
Chapter12
Iron Metabolism in Brucella
R.Martin Roop II, Bryan H.Bellaire, Eric Anderson and James
T.Paulley
236
Chapter13 Brucella Interaction with Membrane Lipids of the Host Cell
Masahisa Watarai 257
Chapter14
Invasion, Intracellular Trafficking and Replication of Brucella
Organisms in Professional and Non-Professional Phagocytes
Edgardo Moreno and Jean-Pierre Gorvel
280
Chapter15
The Intramacrophagic Environment of Brucella spp. and Their
Replicative Niche
Véronique Jubier-Maurin, Séverine Loisel, Jean-Pierre Liautard, and
Stephan Köhler
307
Chapter16
Host Cellular Immune Responses Against Brucella spp. Evaluated
Using the Mouse Model
Cynthia L.Baldwin and Radhika Goenka
335
Chapter17
Development of New Brucella Vaccines by Molecular Methods
David L.Hoover, Mikeljon P.Nikolich, Mina J.Izadjoo, Richard
H.Borschel, and Apurba K.Bhattacharjee
362
Chapter18
Alternative Ovine Brucellosis Vaccine: Experiences with Drug
Delivery Systems
Carlos Gamazo, Maite Estevan, Maribel Murillo, M.Jesús Grilló,
Clara M.Marín, Montserrat Barberán, M.Mar Goñi, José M.Blasco,
and Juan M.Irache
393
Index 418