Biotechnology and Recombinant DNA
Genetic Engineering
Genetic Engineering
Making a Gene Product
Making a Gene Product
E. coli
Used because it is easily grown and its genomics are known
Need to eliminate endotoxin from products
Cells must be lysed to get product
Making a Gene Product
Therapeutic Applications
Subunit vaccines
Nonpathogenic viruses carrying genes for pathogen's antigens as vaccines
Gene therapy to replace defective or missing genes
Human Genome Project
Nucleotides have been sequenced
Human Proteome Project may provide diagnostics and treatments