Parameters Affecting Gene Targeting in Embryonic Stem Cells Dr. Thorsten Buch, Neuroimmunology Thorsten.Buch@neuroimm.unizh.ch 635 3709
Program
Embryonic stem cells
Gene targeting in embryonic stem cells – the method
Targeting by replacement vectors
Targeting by insertion vectors
Identification of recombinants: PCR, Southern-Blot, real-time PCR
Making of the mouse
Factors influencing a targeting experiment
The Paper
The Question
How is it possible to modify the genome of the mouse (or other mammals) at a specific, defined location?
