Introduction 1
Seed Lot System 1
Cryoprotective Agents 2
Preparation of Cells 2
Equilibration . 4
Ampoules and Vials 4
Rate of Cooling 4
Storage 5
Reconstitution (Thawing) 6
Determination of Recovered Cells . 6
Inventory Control . 6
Culture Collection Practices 7
Safety Considerations . 7
Step-by-Step . 8
Selected References . 8
