Contents
Preface ..v
Contributors ..xi
1 Technical Considerations in Quantitating Gene Expression
Richard A. Shimkets  1
2 Gene Expression Quantitation Technology Summary
Richard A. Shimkets  7
3 Standardized RT-PCR and the Standardized Expression
Measurement Center
James C. Willey, Erin L. Crawford, Charles R. Knight, K. A. Warner,
Cheryl A. Motten, Elizabeth A. Herness, Robert J. Zahorchak,
and Timothy G. Graves . 13
4 GeneCalling: Transcript Profiling Coupled to a Gene Database
Query
Richard A. Shimkets . 43
5 Invader Assay for RNA Quantitation
Marilyn C. Olson, Tsetska Takova, LuAnne Chehak, Michelle L. Curtis,
Sarah M. Olson, and Robert W. Kwiatkowski .. 53
6 Monitoring Eukaryotic Gene Expression Using Oligonucleotide
Microarrays
Jennifer Lescallett, Marina E. Chicurel, Robert Lipshutz,
and Dennise D. Dalma-Weiszhausz .. 71
7 Amplified Differential Gene Expression Microarray
Zhijian J. Chen and Kenneth D. Tew .. 95
8 Suppression Subtractive Hybridization
Denis V. Rebrikov, Sejal M. Desai, Paul D. Siebart,
and Sergey A. Lukyanov  107
9 Small Amplified RNA-SAGE
Catheline Vilain and Gilbert Vassart  135
10 Gene Expression Informatics
Martin D. Leach .. 153
Index  167