Part I. Introduction

Cell Migration: An Overview
Donna J. Webb, Huaye Zhang, and Alan F. Horwitz

Part II. Basic Cell Migration and Related Assays

Boyden Chamber Assay
Hong-Chen Chen

Wound Healing Assay
Luis G. Rodriguez, Xiaoyang Wu, and Jun-Lin Guan

Analysis of Cell Migration Using the Dunn Chemotaxis Chamber and Time-Lapse Microscopy
Claire M. Wells and Anne J. Ridley

Cell Adhesion Assays
Dennis F. Kucik and Chuanyue Wu

Cell Spreading Assays
Allison L. Berrier and Susan E. LaFlamme

Cell Scatter Assay
Hong-Chen Chen

Cell Migration Analyses within Fibroblast-Derived 3-D Matrices
Edna Cukierman

Part III. Analysis of Cell Migration in Specialized Cell Systems

Tumor Cell Invasion Assays
Leslie M. Shaw

Analysis of Endothelial Cell Migration Under Flow
Song Li

Angiogenesis Assays in the Chick CAM
Chris Storgard, David Mikolon, and Dwayne G. Stupack

Investigations of Neuronal Migration in the Central Nervous System
Michael E. Ward and Yi Rao

Part IV. Analysis of Cell Migration in Model Organisms

Analysis of Cell Migration in C. elegans
M. Afaq Shakir and Erik A. Lundquist

Analysis of Cell Migration Using Drosophila as a Model System
Jocelyn A. McDonald and Denise J. Montell

Imaging Cell Movement During Dorsal Closure in Drosophila Embryos
William Wood and Antonio Jacinto

Analysis of Cell Movements in Zebrafish Embryos: Morphometrics and Measuring Movement of Labeled Cell Populations In Vivo
Diane S. Sepich and Lilianna Solnica-Krezel

The Xenopus Embryo as a Model System for Studies of Cell Migration
Douglas W. DeSimone, Lance Davidson, Mungo Marsden, and Dominique Alfandari

Neural Crest Migration Methods in the Chicken Embryo
Maria Elena de Bellard and Marianne Bronner-Fraser

In Vivo and In Vitro Models of Mammalian Angiogenesis
Mien V. Hoang and Donald R. Senger

Part V. Biochemical and Novel Approaches in Cell Migration

Measurement of Protein Tyrosine Phosphorylations in Cell Adhesion
Vidhya V. Iyer, Christoph Ballestrem, Joachim Kirchner, Benjamin Geiger, and Michael D. Schaller

Cell Adhesion Assays: Fabrication of an E-cadherin Substratum and Isolation of Lateral and Basal Membrane Patches
Frauke Drees, Amy Reilein, and W. James Nelson

Application of microscope-based FRET to Study Molecular Interactions in Focal Adhesions of Live Cells
Christoph Ballestrem and Benjamin Geiger

Use of Multiphoton Imaging for Studying Cell Migration in the Mouse
Andrea Flesken-Nikitin, Rebecca M. Williams, Warren R. Zipfel, Watt W. Webb, and Alexander Yu

Using Microfluidic Channel Networks to Generate Gradients for Studying Cell Migration
Daniel S. Rhoads, Sharvari M. Nadkarni, Loling Song, Camilla Voeltz, Eberhard Bodenschatz, and Jun-Lin Guan